"Cryopreservation: Applications and Challenges" ed. by Marian Quain, Robert Koprowski
ITexLi | 2023 | ISBN: 1837680272 9781837680276 1837680264 9781837680269 1837680280 9781837680283 | 147 pages | PDF | 5 MB
ITexLi | 2023 | ISBN: 1837680272 9781837680276 1837680264 9781837680269 1837680280 9781837680283 | 147 pages | PDF | 5 MB
This book provides a comprehensive overview of cryopreservation and its applications. It discusses advancements in the field, challenges, guidelines, and recommendations for successful germplasm conservation. Chapters discuss the use of CPAs, cryopreservation of fish sperm, cryopreservation of oocytes and sperms, female fertility preservation, cryopreservation of large structures and tissues, and much more.
Cryopreservation is the preservation of cells at sub-zero temperatures. Although useful, the process is not without its challenges. One of the major challenges is the formation of ice crystals in the cells to be preserved. However, vitrification, where glass is formed instead of crystals, can be achieved using cryoprotection agents (CPAs).
Contents
1. Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications
2. The Current Status of Semen and Oocytes Cryopreservation
3. Female Fertility Preservation: Different Interventions and Procedures
4. Ovarian Tissue Cryopreservation Guidelines
5. Scaling up Cryopreservation from Cell Suspensions to Tissues: Challenges and Successes
6. Impact of Different Cooling Methods on the Stability of Peripheral Blood Mononuclear Cells (PBMCs)
7. Plant Cryopreservation Importance, Approaches and Future Trends
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