Protocols for Studying Pathogenic Fungi
Protocols for Studying Pathogenic Fungi
English | 2025 | ISBN: 9819670462 | 447 Pages | PDF EPUB (True) | 22 MB
English | 2025 | ISBN: 9819670462 | 447 Pages | PDF EPUB (True) | 22 MB
This book is a compilation of protocols for conducting experiments with a variety of yeasts, including S. cerevisiae, Candida albicans, non-albicans Candida sp., Cryptococcus sp.; and molds, including Aspergillus sp., Fusarium sp., Mucor, Rhizopus. The chapters not only discuss National Committee for Clinical Laboratory Standards (NCCLS) accredited protocols but also emphasize the significance of Biosafety Level-2 (BSL-2) facilities. Subsequent chapters cover the essentials of handling, culturing, preserving, and reviving various fungal pathogens. Moreover, advanced techniques are explored, including counting colony-forming units (CFU), growth curve preparation, and microtiter plate assays. A separate chapter discusses the protocols for disc-diffusion, spot and kill-kinetics assays, addressing analyses like a checkerboard and Fractional Inhibitory Concentration (FIC) index assessments. Additionally, the book covers the formation of biofilm, capsule and melanin, fungal cell adhesion, and sterols extraction. Finally, it elaborates on the optimized impact achieved by assessing mitochondrial fitness through non-fermentable carbon.